1) Ultraviolet damage detection with enzymatic and immunologic methods
(André Passaglia Schuch, USP, Brazil).
2) Analysis of DNA methylated sequences
(Monica Capetta and Bernardo Bertoni, Facultad de Medicina, Uruguay)
3) Analysis of DNA content, cell cycle and apoptosis by flow cytometry
(Gustavo A. Folle, IIBCE, Uruguay)
4) Specific DNA lesion detection by Comet Assay
(Graciela Spivak, Stanford University, USA)
5) Molecular markers for translesion synthesis
(Vanesa Gottifredi, Leloir Institute, Argentina).
6) Analysis of acetylated histones by mass spectrometry
(Rosario Duran and Carlos Battiani, IPMONT, Uruguay).
7) Generating chromatin capture conformation (4C) libraries
(J.R. Sotelo, IIBCE, Uruguay).
8) Protein extraction methodologies for histones enrichments
(Wilner Martínez-López, IIBCE, Uruguay)
9) Analysis of gH2AX foci by epifluorescence and confocal microscopy
(Leticia Méndez-Acuña and María Vittoria Di Tomaso, IIBCE, Uruguay)
10) Analysis of chromosomal aberrations by FISH using human and hamster DNA probes. Comparative genome hybridization (CGH)
(Marcelo Larramendy, La Plata University, Argentina)
Two complete cell culture units including 3 biosafety laminar flows, 2 CO2 incubators, 2 inverted microscopes, centrifuges, microcentrifuge, porator and 2 liquid nitrogen cell repositories.
Three epifluorescence microscopes with complete filter sets (in collaboration with the Genetics Section of the Faculty of Sciences).
A complete metaphase finder microscope system including a complete set of filters for epifluorescence studies and appropriate softwares for FISH and Comet assay analyses.
Real Time PCR and MicroArray analyzer.
Three flow cytometers: FACSVantage and MoFlo cell sorters as well as a Cyan analyzer (in collaboration with the Pasteur Institute at Montevideo).
Two Mass Spectrometer: 4800 MALDI TOF/TOF Analyzer (Abi Sciex) and LTG Velos + ETD (Thermo) from Pasteur Institute at Montevideo.
